Introduction Service items Case submission Charges Instruments Training People Research Chinese
 Charges
Package service changing schemes (unit NT$)
 
Item # Item name Deluxe Basic SEC‐MALS only
CF-01 SDS-PAGE a O O O
CF-02 UV scan & 260/280 O O O
CF-03 Tycho NT.6 b O O  
CF-04 Far-UV CD spectrum O O  
CF-05 DSF Tm screen O O  
CF-06 AUC (SV mode) O    
CF-07 SEC c,d,h O   O
CF-08 SEC-MALS e,f,h O   O
User groups Academic Industrial Academic Industrial Academic Industrial
Per sample 11000 54000 4500 11000 5800 28000
 
a. If satisfactory SDS-PAGE information is provided, i.e., CF-01 is not required, a deduction will be made for academic user (NT$500), industrial user (NT$1000) for each sample.
b. If CF-03 is not required, a deduction will be made for academic user (NT$85), industrial user (NT$250) for each sample.
c. If satisfactory SEC information (eluting peaks with baseline resolution) is available, i.e., CF-07 is not required, a deduction will be made for academic user (NT$1900), industrial user (NT$7600) for each sample.
d. Three GE 10/300 analytical grade SEC columns are available: Superdex 75, Superdex 200, and Superose 6.
e. The user must provide the running buffer (1 liter). If the background scattering signal cannot reach 50 µV or lower after overnight equilibration, the calculated molecular weights will have significant errors.
f. Before each sample injection, a standard (BSA) is used to validate the system (with an error within 5%). If replicated measurements are needed, additional charges will apply. Please confirm with ASPC.
g. Service charges include data analysis and processing costs. If the sample composition is complex, an additional processing fee of NTD 800 per hour for experimental data analysis will be charged, and billing will be based on the total hours completed for the case.
h. The user is responsible for preparing the running buffer.
&. When the number of samples is large, entrustment project must be used.
Estimated fee required for determining a protein crystal structure (unit NT$)
 
Service items * AS users Non-AS academic users
PX-01.3 Crystallization screening # 14000 (10 plates) 14000 (10 plates)
PX-02 Incubator for crystal growth 1500 (6 months) 1500 (6 months)
PX-03 Crystal inspection with UV-imaging system 1000 (10 conditions) 1000 (10 conditions)
PX-04.1 Crystal refinement, soaking & co-crystallization 600 (2 plates) 600 (2 plates)
PX-05 Stock solutions 900 (3 units) 900 (3 units)
PX-06 X-ray data collection
PX-06.1 Crystal shipping
PX-06.2 X-ray diffraction test
PX-06.3 X-ray data collection 		 
600 (2 pucks)
1500 (3 conditions)
6000 (3 conditions) 		 
600 (2 pucks)
1500 (3 conditions)
6000 (3 conditions)
PX-07 Structure determination, refinement & validation
PX-07.1 Crystal structure determination
PX-07.2 Model building, refinement & validation 		 
1200 (2 hr)
4200 (7 hr) 		 
1200 (2 hr)
4200 (7 hr)
Estimated fee (NTD) 32,100 32,100
 
* The service charges listed above is based on structure determination of a 40 kDa protein by molecular replacement. The actual fees may vary depending on the difficulty and complexity of the project, especially for the service items PX-01, PX-04, PX-06 and PX-07. The listed charges represent typical steps from protein crystallization, optimization, data collection to structure determination. However, there is no guarantee that a crystal structure can be solved for every case.Project-based service is also available and recommended for users who need full assistance throughout the structure determination processing.
# Users who are not on a project-based service are asked to inspect protein crystals on a regular basis by themselves.
Single service item changing schemes (unit NT$)
 
Item # Item name Academic users Industrial users
Basic protein quality control g
CF-04 Far-UV CD spectrum 600 3000
CF-04.1 Far-UV CD thermal melt (single wavelength) 1000 4500
CF-05 DSF Tm screen 4000 6000
CF-06 AUC (SV mode) 2000 16000&
CF-07 SEC h 2300 9000
CF-08 SEC-MALS h 3600 22000
CF-09 AF4-MALS R&D h 10000 48000
CF-09.1 AF4-MALS Routine h 8200 32000
Protein X-ray crystallization related items
PX-01 Robotic crystallization screening with 96-well plate (per plate) i
PX-01.1 Sitting-drop plate with 1 drop/per well (TW brand) 1100 3000
PX-01.2 Sitting-drop plate with 1 drop/per well (Greiner) 1600 3600
PX-01.3 Sitting-drop plate with 3 drops/per well (Intelli-plate) 1400 3300
PX-01.4 Additive screening with sitting-drop plate (TW brand) 1100 3000
PX-01.5 Additive screening with sitting-drop plate (Intelli-plate) 1400 3300
PX-01.6 Screening with user-prepared plate 1050 2800
PX-01.7 Screening with user-prepared conditions j 1100 2900
PX-01.8 Screening with user-prepared plate and conditions 700 2600
PX-02 Incubators for crystallization plates (plate/month) k 100 300
PX-03 Crystal inspection with UV-imaging system (per condition) l 100 500
PX-04 Crystal refinement, soaking and co-crystallization (per plate) m
PX-04.1 24-well hanging-drop plate (TW brand) 300 1200
PX-04.2 24-well hanging-drop plate without sealant (int’l brand) 350 1300
PX-04.3 24-well hanging-drop plate with sealant (int’l brand) 450 1400
PX-04.4 24-well sitting-drop plate (int’l brand) 350 1300
PX-04.5 48-well sitting-drop plate (int’l brand) 450 1400
PX-04.6 96-well sitting-drop plate (int’l brand) 450 1400
PX-05 Stock solutions for crystallization condition optimization (per unit) 300 500
PX-06 X-ray data collection
PX-06.1 Crystal shipping to synchrotron radiation center (per puck) n 400 Collaboration
PX-06.2 X-ray diffraction test with synchrotron light source (per condition) o 600 Collaboration
PX-06.3 X-ray data collection with synchrotron light source (per set) p 2500 Collaboration
PX-07 Structure determination, model building, refinement and validation (per hour)
PX-07.1 Crystal structure determination q 800 Collaboration
PX-07.2 Model building, refinement and validation for X-ray crystallographic data q 800 Collaboration
PX-07.3 Model building for cryo-EM data r 800 Collaboration
PX-08 Integrated service (from purified protein to validated crystal structure) s Collaboration Collaboration
Customization service
CS-01 Recombinant protein production s Customized Customized
CS-02 Protein crystallization analysis Customized Customized
CS-03 Hydrogen-deuterium exchange mass spectrometry (HDX-MS) data analysis Customized Customized
CS-04 Experimental designs Customized Customized
CS-05 Data analysis Customized Customized
CS-06 Protein structure determination t Collaboration Collaboration
CS-07 Protein function analysis t Collaboration Collaboration
CS-08 Protein dynamics analysis t Collaboration Collaboration
 
h. The additional samples need to be submitted together with the first sample, and the buffer conditions are the same for all the samples. The user is responsible for preparing the running buffer.
i. A pre-crystallization test (PCT) of protein concentration is included. Free for 3-month storage of the plates in our incubators.
j. The facility provides the sitting-drop Intelli-plate.
k. User gets 3-month free storage if the plates are set up via the core service.
l. Only the UV-compatible plates are allowed.
m. The facility provides cover slides, tape and other relevant tools, but not crystallization solutions.
n. Each puck can be loaded with up to 16 crystals.
o. User should pay the fee of crystal shipping.
p. User should upload the preliminary diffraction data for full data set collection and pay the fee of crystal shipping.
q. User should upload the crystal picture, X-ray diffraction image and data-collection statistics and the facility has the right to decide whether a case will proceed.
r. The facility has the right to decide whether a case will proceed.
s. User must provide manpower and pay the usage fee for all the fee-based service items.
t. User must pay manpower costs and the usage fee for all the fee-based service items.
● Charges of ASPC service
*A note for Academia Sinica users: if the payment source comes from non-AS projects, the rate of charges will follow the non-AS academia users according to the official document, No.1090502588, announced by Academia Sinica on 2020/04/10.

The charges are approved by the ASPC user committee on 2021/11/10. This charge might be adjusted according to the operational status.
 

Contact： Ms. Chia-Wei Cheng, Academia Sinica Protein Clinic   Email:ASproteinclinic@gmail.com

No. 128, Sect. 2, Academia Rd., Nankang, Taipei 115, Taiwan (R.O.C.)      TEL：(02) 27855696 #4011

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